Effects of exposure to food contaminated with PBDE, PCN or PCB on reproduction, liver morphology and cytochrome P450 activity in the three-spined stickleback, Gasterosteus aculeatus
Identifieur interne : 001414 ( Main/Exploration ); précédent : 001413; suivant : 001415Effects of exposure to food contaminated with PBDE, PCN or PCB on reproduction, liver morphology and cytochrome P450 activity in the three-spined stickleback, Gasterosteus aculeatus
Auteurs : Gisela Holm [Suède] ; Leif Norrgren [Suède] ; Tommy Andersson [Suède] ; Anders Thurén [Suède]Source :
- Aquatic Toxicology [ 0166-445X ] ; 1993.
Abstract
Female three-spined sticklebacks were fed with freeze-dried chironomids contaminated with low or high doses of polybrominated diphenyl ethers (PBDEs) (Bromkal 70-5DE), polychlorinated naphthalenes (PCNs) (Halowax 1014) and polychlorinated biphenyls (PCBs) (Clophen A50). After 3.5 months of exposure, reproduction studies were started. No significant difference in number of eggs laid was found between the control and exposed groups. Spawning success (considered successful if it occurred within 24 h) in the control group was 80%, whereas it was 20% and 25% in the groups that received high doses of Bromkal 70-5DE or Clophen A50, respectively. After spawning, the fish were dissected for chemical, biochemical and morphological analyses. Levels of PBDE in the low- and high-dose Bromkal 70-5DE groups were 861 ± 271 and 1630 ± 275 mg/kg fat, respectively, whereas the corresponding concentrations of PCN in the Halowax 1014 groups were 845 ± 43 and 1929 ± 72 mg/kg fat, respectively. Concentrations of PCB in fish from the Clophen A50 groups were 1972 ± 158 and 3594 ± 521 mg/kg fat, respectively. Uptake efficiency was approx. 40% for PCB and 20% for PBDE and PCN. Hepatic cytochrome P450-dependent ethoxyresorufin-O-deethylase (EROD) activity was induced by Clophen A50 and Halowax 1014, whereas Bromkal 70-5DE did not significantly induce the enzyme system. EROD activity was 0.8 pmol/mg prot./min in the control group, whereas it was between 2.7 and 16.3 pmol/mg prot./min in the exposed groups. Furthermore, the morphological examination of the liver disclosed pronounced lipid accumulation in all exposed groups.
Url:
DOI: 10.1016/0166-445X(93)90045-3
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000425
- to stream Istex, to step Curation: 000425
- to stream Istex, to step Checkpoint: 000F48
- to stream Main, to step Merge: 001496
- to stream Main, to step Curation: 001414
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Effects of exposure to food contaminated with PBDE, PCN or PCB on reproduction, liver morphology and cytochrome P450 activity in the three-spined stickleback, Gasterosteus aculeatus</title><author><name sortKey="Holm, Gisela" sort="Holm, Gisela" uniqKey="Holm G" first="Gisela" last="Holm">Gisela Holm</name></author><author><name sortKey="Norrgren, Leif" sort="Norrgren, Leif" uniqKey="Norrgren L" first="Leif" last="Norrgren">Leif Norrgren</name></author><author><name sortKey="Andersson, Tommy" sort="Andersson, Tommy" uniqKey="Andersson T" first="Tommy" last="Andersson">Tommy Andersson</name></author><author><name sortKey="Thuren, Anders" sort="Thuren, Anders" uniqKey="Thuren A" first="Anders" last="Thurén">Anders Thurén</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:21E6A85AD67890C986E29225ACB745A25FCBED89</idno><date when="1993" year="1993">1993</date><idno type="doi">10.1016/0166-445X(93)90045-3</idno><idno type="url">https://api.istex.fr/document/21E6A85AD67890C986E29225ACB745A25FCBED89/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000425</idno><idno type="wicri:Area/Istex/Curation">000425</idno><idno type="wicri:Area/Istex/Checkpoint">000F48</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F48</idno><idno type="wicri:doubleKey">0166-445X:1993:Holm G:effects:of:exposure</idno><idno type="wicri:Area/Main/Merge">001496</idno><idno type="wicri:Area/Main/Curation">001414</idno><idno type="wicri:Area/Main/Exploration">001414</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Effects of exposure to food contaminated with PBDE, PCN or PCB on reproduction, liver morphology and cytochrome P450 activity in the three-spined stickleback, Gasterosteus aculeatus</title><author><name sortKey="Holm, Gisela" sort="Holm, Gisela" uniqKey="Holm G" first="Gisela" last="Holm">Gisela Holm</name><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Swedish Environmental Research Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Norrgren, Leif" sort="Norrgren, Leif" uniqKey="Norrgren L" first="Leif" last="Norrgren">Leif Norrgren</name><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Swedish Environmental Research Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Andersson, Tommy" sort="Andersson, Tommy" uniqKey="Andersson T" first="Tommy" last="Andersson">Tommy Andersson</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Zoophysiology, University of Göteborg, Göteborg</wicri:regionArea><wicri:noRegion>Göteborg</wicri:noRegion></affiliation></author><author><name sortKey="Thuren, Anders" sort="Thuren, Anders" uniqKey="Thuren A" first="Anders" last="Thurén">Anders Thurén</name><affiliation wicri:level="3"><country xml:lang="fr">Suède</country><wicri:regionArea>Swedish Environmental Research Institute, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Aquatic Toxicology</title><title level="j" type="abbrev">AQTOX</title><idno type="ISSN">0166-445X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="33">33</biblScope><biblScope unit="page" to="50">50</biblScope></imprint><idno type="ISSN">0166-445X</idno></series><idno type="istex">21E6A85AD67890C986E29225ACB745A25FCBED89</idno><idno type="DOI">10.1016/0166-445X(93)90045-3</idno><idno type="PII">0166-445X(93)90045-3</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-445X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Female three-spined sticklebacks were fed with freeze-dried chironomids contaminated with low or high doses of polybrominated diphenyl ethers (PBDEs) (Bromkal 70-5DE), polychlorinated naphthalenes (PCNs) (Halowax 1014) and polychlorinated biphenyls (PCBs) (Clophen A50). After 3.5 months of exposure, reproduction studies were started. No significant difference in number of eggs laid was found between the control and exposed groups. Spawning success (considered successful if it occurred within 24 h) in the control group was 80%, whereas it was 20% and 25% in the groups that received high doses of Bromkal 70-5DE or Clophen A50, respectively. After spawning, the fish were dissected for chemical, biochemical and morphological analyses. Levels of PBDE in the low- and high-dose Bromkal 70-5DE groups were 861 ± 271 and 1630 ± 275 mg/kg fat, respectively, whereas the corresponding concentrations of PCN in the Halowax 1014 groups were 845 ± 43 and 1929 ± 72 mg/kg fat, respectively. Concentrations of PCB in fish from the Clophen A50 groups were 1972 ± 158 and 3594 ± 521 mg/kg fat, respectively. Uptake efficiency was approx. 40% for PCB and 20% for PBDE and PCN. Hepatic cytochrome P450-dependent ethoxyresorufin-O-deethylase (EROD) activity was induced by Clophen A50 and Halowax 1014, whereas Bromkal 70-5DE did not significantly induce the enzyme system. EROD activity was 0.8 pmol/mg prot./min in the control group, whereas it was between 2.7 and 16.3 pmol/mg prot./min in the exposed groups. Furthermore, the morphological examination of the liver disclosed pronounced lipid accumulation in all exposed groups.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Holm, Gisela" sort="Holm, Gisela" uniqKey="Holm G" first="Gisela" last="Holm">Gisela Holm</name></region><name sortKey="Andersson, Tommy" sort="Andersson, Tommy" uniqKey="Andersson T" first="Tommy" last="Andersson">Tommy Andersson</name><name sortKey="Norrgren, Leif" sort="Norrgren, Leif" uniqKey="Norrgren L" first="Leif" last="Norrgren">Leif Norrgren</name><name sortKey="Thuren, Anders" sort="Thuren, Anders" uniqKey="Thuren A" first="Anders" last="Thurén">Anders Thurén</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Eau/explor/LotaV3/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001414 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001414 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Eau
|area= LotaV3
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:21E6A85AD67890C986E29225ACB745A25FCBED89
|texte= Effects of exposure to food contaminated with PBDE, PCN or PCB on reproduction, liver morphology and cytochrome P450 activity in the three-spined stickleback, Gasterosteus aculeatus
}}
| This area was generated with Dilib version V0.6.39. |  |